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| Kuana,Suzete Lora; Santos,Luciana Ruschel dos; Rodrigues,Laura Beatriz; Borsoi,Anderlise; Moraes,Hamilton Luis do Souza; Salle,Carlos Tadeu Pippi; Nascimento,Vladimir Pinheiro do. |
| The aim of this study was to assess the antimicrobial susceptibility of 62 Campylobacter spp. strains obtained from broiler flocks using the agar diffusion method. The Campylobacter spp strains were isolated from 22 flocks aged between 3 and 5 weeks of life, isolated from cloacae swabs, stools and cecal droppings in the farm and from the carcass rinsing in the slaughterhouse. Campylobacter spp strains were tested on Mueller-Hilton (MH) agar (27 samples) and MH plus TTC agar (35 samples). The antimicrobial susceptibility test revealed a 62.5% resistance to at least one drug, especially to enrofloxacin (71%), neomycin (50%), lincomycin (50%), tetracycline (43%), penicillin (42%), ceftiofur (33%) amoxicillin (27%), spiramycin (20%), ampicillin (18%) and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial resistance; Broiler flocks. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400026 |
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| Borsoi,Anderlise; Santos,Luciana Ruschel do; Rodrigues,Laura Beatriz; Moraes,Hamilton Luiz de Souza; Salle,Carlos Tadeu Pippi; Nascimento,Vladimir Pinheiro do. |
| Over the years, Salmonella Heidelberg (SH) has gained prominence in North America poultry production and in the poultry production of other countries. Salmonella Heidelberg has been isolated and reported from poultry and poultry products in Brazil since 1962, whereas Salmonella Enteritidis (SE) has only emerged as a serious problem in poultry and public health since 1993. These strains of Salmonella can cause intestinal problems in newly hatched chicks, and infection may persist until adulthood. Upon slaughter of chickens, Salmonella can contaminate carcasses, a condition that poses a threat to human health. The aim of this study was to compare the fecal excretion of Salmonella Enteritidis and Salmonella Heidelberg in newly hatched chicks (orally... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella Heidelberg; Salmonella Enteritidis; Fecal Excretion; Villus/crypt ratio; Broiler. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100034 |
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| Gonsalves,Camila Cristina; Borsoi,Anderlise; Perdoncini,Gustavo; Rodrigues,Laura Beatriz; Nascimento,Vladimir Pinheiro do. |
| ABSTRACT Campylobacter spp. cause foodborne illnesses in humans primarily through the consumption of contaminated chicken. The aim of this study was to evaluate the United States Department of Agriculture's (USDA) recommended methodology, protocol MLG 41.02, for the isolation, identification and direct plate counting of Campylobacter jejuni and C. coli samples from the broiler slaughtering process. A plating method using both mCCDA and Campy-Cefex agars is recommended to recover Campylobacter cells. It is also possible to use this method in different matrices (cloacal swabs and water samples). Cloacal swabs, samples from pre-chiller and post-chiller carcasses and samples of pre-chiller, chiller and direct supply water were collected each week for four... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter; Agar plate count; MCCDA; Campy-Cefex; Broiler. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300764 |
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| Pilotto,Fernando; Klein,Vilson Antonio; Wald,Vera Beatriz; Rodrigues,Laura Beatriz; Santos,Luciana Ruschel dos; Colussi,Frederico de Mello; Nascimento,Vladimir Pinheiro do. |
| O piso de chão batido é utilizado na maioria das granjas avícolas brasileiras devido ao baixo custo em relação à construção de pisos concretados. A presença de matéria orgânica na superfície do solo dificulta sua desinfecção, devido à redução da ação antimicrobiana dos desinfetantes, sendo que os testes realizados in vitro para verificar a eficácia dos desinfetantes não contemplam as condições adversas encontradas em granjas avícolas. Assim, o presente trabalho objetivou avaliar a contaminação por coliformes totais e fecais através do número mais provável (NMP) no piso de chão batido de uma granja avícola e definir o volume de solução desinfetante necessário para saturar uma determinada área conforme a composição físico-hídrica do solo. Foi detectada a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aves; Coliformes; Desinfecção; Piso de chão batido; Solo. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000600026 |
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| Vancin,Franciele Rampazzo; Webber,Bruna; Bondan,Carlos; Daroit,Luciane; Santos,Luciana Ruschel dos; Rodrigues,Laura Beatriz. |
| ABSTRACT: The preservation of milk samples for microbiological analyses by the Brazilian Network of Milk Quality Control Laboratories requires the addition of preservatives to maintain the microbiota from the time of sample collection to the moment of analysis. The number of microorganisms can change as a result of the active ingredients and concentration of the preservative, as well as due to interactions between the preservatives, incubation time, and packaging temperature. The objective of this research was to evaluate the conservation potential of different concentrations of sodium azide and chloramphenicol on the analytical shelf life of milk samples. Two farms were selected, one with a low bacterial count and one with a high bacterial count. The milk... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Milk; Azidiol; Individual bacterial count; Flow cytometry. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000200750 |
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| Basso,Paula Cristina; Barcellos,Heloísa Helena Alcântara; Brun,Maurício Veloso; Rodrigues,Laura Beatriz; Bortolini,Carlos Eduardo; Melatti,Luciane; Scalco Neto,João Francisco; Bastiani,Paulo Vinícius; Valle,Stella de Faria; Santos,Luciana Ruschel dos. |
| Com o objetivo de se avaliarem os resultados do uso de endoscopia rígida e da técnica por tubo endotraqueal de colheita de líquido traqueobrônquico, foram utilizados 28 cães, errantes ou domiciliados, distribuídos em dois grupos. Nos cães do grupo 1, aplicou-se a técnica de lavado traqueobrônquico em 14 cães, sendo sete sadios e sete com sinais clínicos respiratórios. Nos do grupo 2, utilizou-se a mesma técnica, entretanto com endoscopia rígida, em sete cães sadios e em sete cães com sinais clínicos de doença respiratória. Os dados evidenciaram que o procedimento utilizando-se endoscópio rígido resultou em menor volume de solução infundida e em maior porcentagem de solução recuperada. Não houve diferença significativa quanto ao tempo de duração da execução... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lavagem traqueobrônquica; Tubo endotraqueal; Endoscopia rígida; Cão. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000300020 |
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| Rodrigues,Laura Beatriz; Santos,Luciana Ruschel dos; Tagliari,Vinícius Zancanaro; Rizzo,Natalie Nadin; Trenhago,Graciela; Oliveira,Amauri Picollo de; Goetz,Franciane; Nascimento,Vladimir Pinheiro do. |
| This study assessed biofilm formation on polystyrene by Staphylococcus aureus, Listeria monocytogenes, L. welshimeri and Escherichia coli, isolated from a slaughtering plant, grown on tryptic soy broth (TSB) using different glucose concentrations. The tested bacteria produced biofilm in at least one of the concentrations used, and some of them were strong biofilm producers. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Listeria; Escherichia coli; Staphylococcus aureus; Biofilm; Polystyrene. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400029 |
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| Webber,Bruna; Oliveira,Amauri Picollo de; Pottker,Emanuele Serro; Daroit,Luciane; Levandowski,Rafael; Santos,Luciana Ruschel dos; Nascimento,Vladimir Pinheiro do; Rodrigues,Laura Beatriz. |
| ABSTRACT: We evaluated the influence of temperature on the ability of Salmonella Enteritidis (SE) to form biofilms on stainless steel, polyethylene, and polyurethane surfaces under different hygiene procedures. These materials were placed on SE culture and incubated at 42±1 ºC, 36±1 ºC, 25±1 ºC, 9±1 ºC, and 3±1 ºC for 4, 8, 12, and 24 h. Hot water at 45 ºC and 85 ºC, 0.5% peracetic acid solution, and 1% quaternary ammonia were used for hygienization. Biofilm formation occurred at all temperatures evaluated, highlighting at 3 ºC which has not been reported as an ideal temperature for the adhesion of SE to these materials. The SE adhered more often to polyethylene surfaces than to polyurethane and stainless steel surfaces (P<0.05). Peracetic acid and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella Enteritidis; Biofilms; Surfaces; Hygiene procedures; Food microbiology. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000700452 |
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